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Profiling the occurrence of biogenic amines in different types of tuna samples using an improved analytical approach
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Advisor(s)
Abstract(s)
Food deterioration caused by microbial agents often involve the formation of biogenic amines (BAs), which can
have harmful effects on human health. In this study a set of BAs - tryptamine, cadaverine, putrescine, spermine,
histamine, tyramine, and spermidine, were simultaneously analysed to monitor their occurrence in different
types of tuna samples. An improved extraction approach involving ultrasound-assisted microextraction (USAμET)
followed by derivatization with dansyl chloride (DnsCl) and analysis by ultrahigh performance liquid chroma tography (UHPLC) with fluorescence detection was validated for BAs quantification. The performance of the
USAμET/UHPLC-FLD was assessed by studying the limits of detection (LOD) and quantification (LOQ), linear
dynamic range (LDR), precision (intra and inter-day) and matrix effect (ME). Good linearity (r2 > 0.98), LODs
(from 0.98 to 8.57 mg kg− 1
) and LOQs (3.20–25.6 mg kg− 1
) were achieved for all BAs analysed. Recoveries
ranged from 76% to 106%, with relative standard deviations (RSD) lower than 5.0%. ME was determined from
7.52 to 50% and the intra and inter-day precisions ranged from 4.7 to 11.6% and 5.5–14.2%, respectively. BAs
levels varied significantly from 4.09 ± 0.8 mg kg− 1 of putrescine in olive oil canned samples to 577.9 ± 5.9 mg
kg− 1 of cadaverine in natural canned samples. Tryptamine and tyramine were not detected in any of the samples
analysed, while spermine and spermidine were found in 85.7% of the assayed tuna samples. Cadaverine was the
most dominant BA with concentrations ranging from 54.3 ± 2.5 mg kg− 1
, in olive oil canned tuna, to 577.9 ±
5.9 mg kg− 1
, in natural canned samples, whereas putrescine had the lowest concentration (average 6.9 ± 2.5 mg
kg− 1
). The validated methodology revealed important improvements in terms of simplification of the experi mental layout, expressed in the low sample and reagent amounts, in addition to less time-consuming and labour intensive requirements that did not compromise the analytical performance.
Description
Keywords
Biogenic amines Tuna samples USAμET DnsCl UHPLC-FLD Food safety . Faculdade de Ciências Exatas e da Engenharia Faculdade de Ciências da Vida Centro de Química da Madeira
Citation
Pataca, J. K., Porto-Figueira, P., Pereira, J. A., Caldeira, H., & Câmara, J. S. (2021). Profiling the occurrence of biogenic amines in different types of tuna samples using an improved analytical approach. LWT, 139, 110804. https://doi.org/10.1016/j.lwt.2020.110804
Publisher
Elsevier