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- Structure and floristic composition associated with an endangered species Beta patula Aiton (Amaranthaceae) in the Islands of Madeira ArchipelagoPublication . Nóbrega, Humberto; Freitas, Gregório; Zavattieri, M. A.; Ragonezi, Carla; Carvalho, Miguel Â. A. Pinheiro deTwenty-two native Crop Wild Relatives (CWR) occur in specific dry environments of Madeira Archipelago, like Desembarcadouro islet in Ponta de São Lourenço and Chão islet in Desertas Islands. Nine of them share the same gene pool with crop species included in Annex I of the International Treaty on Plant Genetic Resources for Food and Agriculture. Amongst them, Beta patula Aiton, an IUCN Critically Endangered species, has been studied in detail for in situ and ex situ conservation. The present paper summarises the information recorded during the Beta patula population assessment. Valuable information on plant communities associated with this species was obtained.
- Phenotyping the ideotypes of two maize landraces from Madeira archipelago using morpho-agronomic traits and zein patternPublication . Carvalho, Miguel Â. A. Pinheiro de; Sardinha, Carmen M. S.; Gouveia, Carla S.S.; Silva, Emanuel M. da; Nóbrega, Humberto G. M.; Ganança, José F. T.; Nóbrega, Sónia R.; Santos, Teresa M. M. dosMadeira Archipelago holds specific maize genetic resources whose populations, representing field diversity, were previously classified into four main landrace groups. The ISOPs 0061 and 0070, with common names of “Milho Sequeiro” and “Milho Branco” yellow and white maize, respectively, represent the ideotypes of two of these landraces. These ideotypes have now been analyzed using morpho-agronomic and biochemical traits, to phenotype these landraces. The variation of nine of the ten morpho-agronomic traits was used to separate the landraces ideotypes. However, the seven traits of seed biochemical composition did not allow to segregate the landraces ideotypes. This is not the case of zein pattern, which polymorphism shows to be specific for each ideotype. The original ideotypes populations were regenerated twice under low input conditions. Zein patterns of the original and regenerated accessions were compared, using two electrophoresis techniques. The lab-on-a-chip electrophoresis showed that the standard SDS-PAGE zein pattern with six fractions could be separated into eighteen (ISOP 0061) and twenty (ISOP 0070) protein bands. In contrast, no significant changes were detected in the zein pattern structure of the initial and regenerated accessions of both landraces ideotypes. The chip electrophoresis showed to be a suitable technique to screen and characterize a large number of individuals and accessions of the germplasm collections, due to its reproducibility. In particular, the zein patterns can be used to phenotype ideotypes and establish a formula representing their structure, allowing to detect changes in landrace structure, occurring as a result of gene bank management actions.
- Drought avoidance and phenotypic flexibility of sweet potato (Ipomoea batatas (L.) Lam.) under water scarcity conditionsPublication . Gouveia, Carla S. S.; Ganança, José F. T.; Nóbrega, Humberto G. M.; Freitas, José G. R.; Lebot, Vincent; Carvalho, Miguel Â. A. Pinheiro deSweet potato (Ipomoea batatas (L.) Lam.) is an important staple food in several regions of the world. Water scarcity is the most devastating abiotic stress, with a great impact on crop productivity, food security, and subsistence. Drought restricts the nutrient intake and transport into the plant. Tolerant crops have morphological mechanisms of drought avoidance and/or phenotypic flexibility, showing also good water and nutrient efficiency. However, that information is scarce for sweet potato, which is usually based on physiological traits of plant productivity. Here, we show the physiological responses of eight sweet potato accessions subjected to a 3 months’ drought period, by recording their differences for nutrient and leaf chlorophyll content, biomass and stress level. Our results showed that the differences in water use efficiency (WUE, +68.1%), chlorophyll content index (CCI, -5.3%), total plant biomass (TPB, -55.4%), nutrient efficiency (NER, +38.1%) and nutrient harvest index (NHI, +2.9%) where significantly correlated with the water regime. The water shortage led to a drought avoidance response, with TPB loss in all accessions. Distinct phenotypic flexibility responses were also recorded and explained by the root:shoot ratio (R:S) and stress index (SI) variation of the storage root and shoot growth. This information could be relevant for the development of sweet potato breeding programs, adapting this crop to climate change.
- Genetic variability of high molecular weight glutenin subunits in bread wheat from continental Portugal, Madeira and Canary IslandsPublication . Santos, Teresa M. M. dos; Nóbrega, Humberto; Ganança, José Filipe Teixeira; Silva, Emanuel; Afonso, Desire; Gutiérres, Abílio F. M.; Slaski, Jan J.; Khadem, Manhaz; Carvalho, Miguel Â. A. Pinheiro deThe genetic variability of high molecular weight glutenin subunits (HMWGS) composition at the Glu-1 loci in bread wheat (Triticum aestivum L.) was studied electrophoretically using the SDS–PAGE in 3,470 individuals representing 159 populations originated from the Canary Islands (Spain), the Archipelago of Madeira (Portugal) and the continental Portugal. A total of 25 alleles were detected, resulting in 69 different allele combinations. The geographical distribution of the high molecular weight glutenin alleles confirms historical data regarding circulation of wheat germplasm between the Iberian Peninsula and Madeira and between Madeira and the Canary Islands and vice versa.
- Prospeção e caracterização dos recursos genéticos de macieira, Malus domestica Borkh, na MadeiraPublication . Gouveia, Carla S.S.; Ascarini, Fabio; Nóbrega, Humberto G. M. de; Freitas, José G. R. de; Carvalho, Miguel Â. A. Pinheiro deA Madeira possui condições agroecológicas únicas, que permitem o desenvolvimento de grande diversidade de espécies frutícolas. A macieira é uma fruteira tradicional na Madeira que se distribui pelas suas freguesias montanhosas, desde o Oeste, Achadas da Cruz, até o Este, Faial. O cultivo da macieira desenvolve-se em pequenos pomares, que albergam uma diversidade de cultivares, cuja produção é consumida em fresco ou utilizada na produção de sidra. A prospeção e inventariação dos recursos genéticos desta fruteira procura aprofundar os conhecimentos sobre a sua diversidade, tendo resultado no registo de 10 variedades de conservação no Catálogo Nacional de Variedades, entre as quais o Pero Domingos, a Maçã Barral, a Maçã Cara de Dama e o Pero Calhau. Os dados desta inventariação são disponibilizados, através das plataformas GRIN-Global e GBIF. Está em curso um projeto que visa a valorização destas variedades locais, através da sua caracterização morfo-agronómica e nutricional. O presente trabalho apresenta os resultados preliminares da caracterização morfométrica de 4 dessas variedades. Os parâmetros utilizados incluem o hábito de crescimento, época de floração, frutificação e colheita, e 10 caracteres morfológicos do fruto. Os resultados obtidos permitem-nos inferir que as 4 variedades são distintas entre si e apresentam características agronómicas que lhes conferem valor comercial. Adicionalmente, o fruto do Pero Domingos foi analisado em relação a 5 parâmetros nutricionais e físico-químicos, e à sua aptidão para consumo em fresco ou processado (desidratado, compotas, sidra). O Pero Domingos apresentou um bom tempo de conservação no pós-colheita. No entanto, recomenda-se o seu uso na produção de sidra ou confeção de compotas até 1 semana de pós-colheita. O teor de sólidos solúveis variou significativamente nas compotas. O pH da sidra diminuiu significativamente com a profundidade da coluna na pipa.
- Morphological evaluation of common bean diversity on the Island of MadeiraPublication . Freitas, Gregório; Ganança, José F.T.; Nóbrega, Humberto; Nunes, Élvio; Costa, Graça; Slaski, Jan J.; Carvalho, Miguel Â. A. Pinheiro deThe variability of fifty populations of common bean (Phaseolus vulgaris L.), representing a wide range of ecological conditions on the Island of Madeira, was evaluated based on morphological and reproductive traits. Individual data of 58 traits related to earliness, plant and tassel structure and the shape of the ear and grain were analysed using multivariate analysis. The populations belonging to two major common varieties were clustered into fifteen groups by their degree of dissimilarity, based on discriminant analysis. The dissimilarity of these groups was con- firmed by one way ANOVA. The racial rank of these groups was proposed and a brief description of the common bean landraces was presented. This work represents the first morphological characterization and analysis of diversity of bean germplasm from the Archipelago of Madeira, where the traditional agricultural practices are still keeping this Portuguese region free from improved bean varieties. The description of the Madeiran bean landraces allows the preservation of the existing bean biodiversity and could be used for their registration as conservation landraces, or for conservation and breeding purposes worldwide.
- Anticipating the Climate Change Impacts on Madeira’s Agriculture: The Characterization and Monitoring of a Vine AgrosystemPublication . Carvalho, Miguel Â. A. Pinheiro de; Ragonezi, Carla; Oliveira, Maria Cristina O.; Reis, Fábio; Macedo, Fabrício Lopes; Freitas, José G. R. de; Nóbrega, Humberto; Ganança, José Filipe T.Climate—Madeira Strategy (CMS) foresees two models to describe the climate scenarios for the Madeira region in 2050 and 2070. These scenarios anticipate an average temperature rise of 1.4 to 3.7 ◦C and a decrease in precipitation by 30 to 40%. Consequently, Madeira’s agriculture will suffer the impacts of climate change. To understand these impacts, a baseline of major agrosystem components needs to be established, with the ultimate goal to monitor its consequences in its functioning. CASBio project used the 1961–1991 and 2010–2020 meteorological data series to modulate climate conditions and characterize and monitor six agrosystems for 2 years. One of them was a vineyard, Quinta das Vinhas, representing a typical agrosystem in the Mediterranean climate. The annual and seasonal variation in climatic parameters, soil conditions, microbiological communities, floristic and insect diversity, and crop production was assessed, using a total of 50 parameters. The results were used to establish a baseline of the agrosystem components and their seasonal and annual variation. The major findings are: (i) winter and summer extreme events show a trend in temperature and precipitation supporting a fast change in climate; (ii) a critical imbalance between nitrogen fixing and denitrifying bacteria was identified, especially in summer, that could be determined by the rise in temperature and drought; (iii) among floristic diversity, the therophytes and geophytes confirm to be the most suitable indicators for the rise in temperature and reduction in precipitation in the agrosystems; (iv) an imbalance in favor of C. capitata plague was observed, associated with the summer rise in temperature and decrease in precipitation; (v) despite an increase in most of the grape varieties production, the Madeiran wine local varieties were shown to be less stable in productivity under observed climate conditions. The agrosystem baseline is a starting point for long term monitoring and allows for further quantifying the influence of climate change on agrosystem productivity, resilience, and sustainability.
- Phenotypic flexibility and drought avoidance in taro (Colocasia esculenta (L.) Schott)Publication . Gouveia, Carla S. S.; Ganança, José F. T.; Nóbrega, Humberto G. M.; Freitas, José G. R. de; Lebot, Vincent; Carvalho, Miguel Â. A. Pinheiro deTaro (Colocasia esculenta (L.) Schott) is a substantial staple food in most of the tropical regions. Prolonged exposure to drought impairs crop production worldwide. Tolerant crops have the best capability to cope and avoid drought, through phenotypic flexibility mechanisms. The water use efficiency (WUE) is well known in taro crops, but very scarce information is available relating to their nutrient efficiency (NER) in drought conditions. Our work provided pertinent information about the physiological variation of seven taro accessions subjected to seven months of drought, by recording the differences for nutrient allocation, chlorophyll canopy, biomass loss, and stress intensity. Significant relationships between control and drought treatments on WUE (+85%), total plant biomass (TPB, -26.8%), chlorophyll content index (CCI, +1.8%), and nutrient harvest index (NHI, +0.2%) were detected. Drought led to a generalized loss of TPB as drought avoidance strategy, although distinct phenotypic flexibility was observed through the root: shoot ratio (R:S) and stress index (SI) from the corm and shoot organs. The nutrient allocation from the corms to shoots, with NER increase registered in drought conditions, can be a valuable tool to complement the TPB and WUE productivity traits, to be used in taro breeding programs.
- Adapting Agriculture to Climate Change: A Synopsis of Coordinated National Crop Wild Relative Seed Collecting Programs across Five ContinentsPublication . Eastwood, Ruth J.; Tambam, Beri B.; Aboagye, Lawrence M.; Akparov, Zeynal I.; Aladele, Sunday E.; Allen, Richard; Amri, Ahmed; Anglin, Noelle L.; Araya, Rodolfo; Arrieta-Espinoza, Griselda; Asgerov, Aydin; Awang, Khadijah; Awas, Tesfaye; Barata, Ana Maria; Boateng, Samuel Kwasi; Magos Brehm, Joana; Breidy, Joelle; Breman, Elinor; Brenes Angulo, Arturo; Burle, Marília L.; Castañeda-Álvarez, Nora P.; Casimiro, Pedro; Chaves, Néstor F.; Clemente, Adelaide S.; Cockel, Christopher P.; Davey, Alexandra; De la Rosa, Lucía; Debouck, Daniel G.; Dempewolf, Hannes; Dokmak, Hiba; Ellis, David; Faruk, Aisyah; Freitas, Cátia; Galstyan, Sona; García, Rosa M.; Ghimire, Krishna H.; Guarino, Luigi; Harker, Ruth; Hope, Roberta; Humphries, Alan W.; Jamora, Nelissa; Jatoi, Shakeel Ahmad; Khutsishvili, Manana; Kikodze, David; Kyratzis, Angelos C.; León-Lobos, Pedro; Liu, Udayangani; Mainali, Ram P.; Mammadov, Afig T.; Manrique-Carpintero, Norma C.; Manzella, Daniele; Mat Ali, Mohd Shukri; Medeiros, Marcelo B.; Mérida Guzmán, María A.; Mikatadze-Pantsulaia, Tsira; Mohamed, El Tahir Ibrahim; Monteros-Altamirano, Álvaro; Morales, Aura; Müller, Jonas V.; Mulumba, John W.; Nersesyan, Anush; Nóbrega, Humberto G.M.; Nyamongo, Desterio O.; Obreza, Matija; Okere, Anthony U.; Orsenigo, Simone; Ortega-Klose, Fernando; Papikyan, Astghik; Pearce, Timothy R.; Carvalho, Miguel Â. A. Pinheiro de; Prohens, Jaime; Rossi, Graziano; Salas, Alberto; Singh Shrestha, Deepa; Siddiqui, Sadar Uddin; Smith, Paul P.; Sotomayor, Diego A.; Tacán, Marcelo; Tapia, César; Toledo, Álvaro; Toll, Jane; Vu, Dang Toan; Vu, Tuong Dang; Way, Michael J.; Yazbek, Mariana; Zorrilla, Cinthya; Kilian, BenjaminThe Adapting Agriculture to Climate Change Project set out to improve the diversity, quantity, and accessibility of germplasm collections of crop wild relatives (CWR). Between 2013 and 2018, partners in 25 countries, heirs to the globetrotting legacy of Nikolai Vavilov, undertook seed collecting expeditions targeting CWR of 28 crops of global significance for agriculture. Here, we describe the implementation of the 25 national collecting programs and present the key results. A total of 4587 unique seed samples from at least 355 CWR taxa were collected, conserved ex situ, safety duplicated in national and international genebanks, and made available through the Multilateral System (MLS) of the International Treaty on Plant Genetic Resources for Food and Agriculture (Plant Treaty). Collections of CWR were made for all 28 targeted crops. Potato and eggplant were the most collected genepools, although the greatest number of primary genepool collections were made for rice. Overall, alfalfa, Bambara groundnut, grass pea and wheat were the genepools for which targets were best achieved. Several of the newly collected samples have already been used in pre-breeding programs to adapt crops to future challenges.
- Distribution of Genetic Diversity in Beta patula Aiton Populations from Madeira Archipelago, PortugalPublication . Ragonezi, Carla; Nóbrega, Humberto; Leite, Maria Inês; Freitas, José G. R. de; Macedo, Fabrício Lopes; Carvalho, Miguel Â. A. Pinheiro deBeta patula Aiton is a crop wild relative (CWR) which belongs to the Gene Pool 1b and is considered a Critically Endangered species, and is present in very specific environments, such as the Desembarcadouro islet (DI) in Ponta de São Lourenço or Chão islet (CI) in the Desertas Islands. The ISOPlexis Center (University of Madeira) has been providing continuous support for its in situ conservation by keeping a management plan of wild populations and an ex situ conservation strategy through the storage of accessions in the ISOPlexis GeneBank. The present work intends to present the spatial distribution of genetic variability and diversity in these B. patula populations using eight polymorphic Simple Sequence Repeat (SSR) markers. The overall results lead to the identification of three spots with a high genetic diversity. CI with a small cluster of individuals shows a genetic footprint different from DI, having unique alleles present in its population. DI has two distinct areas: the western area, with a higher individual density but with a lower genetic diversity and higher allele fixation; and the central area, with a lower individual count but with a higher genetic diversity and with the presence of unique alleles. Despite some genetic differences, the comparison of the two islets’ DI and CI populations shows that they have more similarities than differences. Analysis of the Molecular Variance, based on the hierarchical cluster, showed a 9% diversity between populations, 68% among individuals, and 23% within individuals. This data will be used for the establishment of a protocol to monitor and manage B. patula genetic diversity under a genetic reserve, subsequently contributing to the European Genetic Reserve network implementation and the protection of this important CWR.